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Addgene inc lego ig2
Lego Ig2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lego+ig2/LeGO-iG2+(Plasmid+%2327341)/pmc12915289-103-0-9
Average 94 stars, based on 64 article reviews
lego ig2 - by Bioz Stars, 2026-09
94/100 stars

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Plasmid Preparation:

Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer.
Article Snippet: Plasmids psPAX2 and pMD2.G were a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260 and Addgene plasmid # 12259; http://n2t.net/ addgene:12259; RRID: Addgene_12259). .. LeGO-iG2 was a gift from Boris Fehse (Addgene plasmid # 27341; http://n2t.net/addgene:27341; RRID: Addgene_27341) (Weber et al, 2008). .. LT3-GEPIR was a gift from Johannes Zuber (Addgene plasmid # 111177; http://n2t.net/ addgene:111177; RRID: Addgene_111177) (Fellmann et al, 2013).

Article Title: HERC5 downregulation in non-small cell lung cancer is associated with altered energy metabolism and metastasis.
Article Snippet: .. LeGO‐iG2 was a gift from Boris Fehse (Addgene plasmid #27341; http:// n2t. net/ addge ne: 27341; RRID: Addgene_27341). ..

Article Title: Translation of numerous upstream open reading frames rather than their products is essential for proliferation of human cells of distinct origin
Article Snippet: .. The plasmid used to validate the screening results in a competition assay (LeGO-gG2) was previously made using LeGO-iG2 (Addgene #27341) and lentiGuide-Puro vector (Addgene #52963) ( ). ..

Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer
Article Snippet: Plasmids psPAX2 and pMD2.G were a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID: Addgene_12260 and Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID: Addgene_12259 ). .. LeGO-iG2 was a gift from Boris Fehse (Addgene plasmid # 27341; http://n2t.net/addgene:27341 ; RRID: Addgene_27341 ) (Weber et al, ). .. LT3-GEPIR was a gift from Johannes Zuber (Addgene plasmid # 111177; http://n2t.net/addgene:111177 ; RRID: Addgene_111177 ) (Fellmann et al, ).

Over Expression:

Article Title: CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator
Article Snippet: .. For overexpression studies, human ZNF740 (NM_001004304) and human NOXA (NM_021127) were cloned into a modified LeGO-iG2 (Addgene 27341) lentiviral vector by replacing EGFP with an EGFP-P2A-Neomycin cassette. ..

Article Title: CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator.
Article Snippet: .. For overexpression studies, human ZNF740 (NM_001004304) and human NOXA (NM_021127) were cloned into a modified LeGO-iG2 (Addgene 27341) lentiviral vector by replacing EGFP with an EGFP-P2A-Neomycin cassette. ..

Clone Assay:

Article Title: CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator
Article Snippet: .. For overexpression studies, human ZNF740 (NM_001004304) and human NOXA (NM_021127) were cloned into a modified LeGO-iG2 (Addgene 27341) lentiviral vector by replacing EGFP with an EGFP-P2A-Neomycin cassette. ..

Article Title: CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator.
Article Snippet: .. For overexpression studies, human ZNF740 (NM_001004304) and human NOXA (NM_021127) were cloned into a modified LeGO-iG2 (Addgene 27341) lentiviral vector by replacing EGFP with an EGFP-P2A-Neomycin cassette. ..

Modification:

Article Title: CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator
Article Snippet: .. For overexpression studies, human ZNF740 (NM_001004304) and human NOXA (NM_021127) were cloned into a modified LeGO-iG2 (Addgene 27341) lentiviral vector by replacing EGFP with an EGFP-P2A-Neomycin cassette. ..

Article Title: CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator.
Article Snippet: .. For overexpression studies, human ZNF740 (NM_001004304) and human NOXA (NM_021127) were cloned into a modified LeGO-iG2 (Addgene 27341) lentiviral vector by replacing EGFP with an EGFP-P2A-Neomycin cassette. ..

Competitive Binding Assay:

Article Title: Translation of numerous upstream open reading frames rather than their products is essential for proliferation of human cells of distinct origin
Article Snippet: .. The plasmid used to validate the screening results in a competition assay (LeGO-gG2) was previously made using LeGO-iG2 (Addgene #27341) and lentiGuide-Puro vector (Addgene #52963) ( ). ..



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